Journal: International Journal of Molecular Sciences
Article Title: Effect of Autophagy Regulated by Sirt1/FoxO1 Pathway on the Release of Factors Promoting Thrombosis from Vascular Endothelial Cells
doi: 10.3390/ijms20174132
Figure Lengend Snippet: ( A ) Viability of HUVEC treated with different concentrations of ox-LDL. ( B ) Concentration of von Willebrand factor (vWF) in media. ( C ) Expression of vWF on cytomembrane. ( D ) Expression of P-selectin on cytomembrane. ( E ) Expression of Sirt1, FoxO1, LC3-II/I and p62. Values are presented as the means ± SD (MTT and ELISA assay: n = 6, flow cytometry and Western Blot: n = 3). Compared with control groups at each time point, * p < 0.05, ** p < 0.01, *** p < 0.001.
Article Snippet: Mouse anti-human Sirt1, FoxO1 and Rab7 antibodies and rabbit anti-human Ac-FoxO1 antibody were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).
Techniques: Concentration Assay, Expressing, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Western Blot, Control